東京大学 大学院農学生命科学研究科
附属食の安全研究センター　栃内研究室

・Comparative analysis of the cardiotoxicity induced by doxorubicin and epirubicin and exploratory investigations of clinical parameters indicating the cardiotoxicity in healthy dogs.

Shotaro Shimano, Hirotaka Tomiyasu*, Kento Ishikawa, Hiroki Sakuma, Hiroto Toyoda, Ryota
Tochinai, James K. Chambers, Kazuyuki Uchida, Takeshi Mizuno, Yuko Goto-Koshino, Masaru
Okuda. J. Vet. Med. Sci. (2026) in press.

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・Altered flagellin-TLR5 signaling pathway in interaction between host and laboratory Escherichia coli strains engineered for enhanced intestinal colonization in mouse.

Tsuyoshi Tachibana†, Atsushi Minami†, Ryutaro Fukui, Ryota Tochinai, Kohei Mochizuki,
Wataru Fujii, Tetsuhiro Ogawa*, Shigeru Kakuta*. Biosci. Biotechnol. Biochem. (2026) zbag079.

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・東京優駿（日本ダービー）を制した葦毛馬の高齢期における心拍変動および病理組織学的検討.

栃内 亮太, 李 俊佑, チェンバーズ ジェームズ, 遠藤 麻衣子, 内田 和幸, 関澤 信一, 桑原 正貴*. Hippophile. (2026) in press.

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・Signal-Averaged Electrocardiography in Horses: Characteristics of Ventricular Late Potentials in Healthy Thoroughbreds and Horses with Cardiac Abnormalities.

Seki N, Tochinai R*, Sekizawa S, Ohmura H, Ueno T, Fukuda K, Kuwahara M. J Equine Sci. (2026) 37(2):41-51.

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・Poly(2-ethyl-2-oxazoline)-Conjugated Porcine Serum Albumin as a Veterinary Albumin Therapeutic.

Fujisawa J, Hattori R, Saito K, Yamada T, Yamaguchi M, Hashimoto R, Kohno M, Tochinai R, Komatsu T*. ACS Appl Bio Mater. (2026) 9(7), 3412–3424.

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・Intravenous administration of a selective metabotropic glutamate receptor 2/3 agonist attenuates hypertension in spontaneously hypertensive rats.

Aoki S, Nagashima Y, Tochinai R, Sekizawa S*. J Vet Med Sci. (2026) 88(5). 719-727.

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・A preliminary examination of short-term tacrolimus treatment for a CRISPR/Cas9-generated rat model with Duchenne muscular dystrophy. 

Yusuke Eiraku†, Ryota Tochinai*†, Koichi Kimura*†, Hiroyuki Morita, Norifumi Takeda, Koki Nakanishi, Kazutoshi Hirose, Jonna Rose C Maniwang, Wataru Fujii, Shigeru Kakuta, Shin-ichi Sekizawa, Masao Daimon, Yoshitsugu Aoki, Norihiko Takeda, and Keitaro Yamanouchi. Transl Regul Sci. (2026) 8(1). 1-6.

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・Real-World Quality Assessment of Medium- and Long-Chain Triglycerides Oil for Deep Fried Japanese Rice Cake (Agemochi).

Yushi Muto, Kazumasu Sasaki, Ryota Tochinai, Yasuko Tatewaki, Tatsushi Mutoh, Yasuyuki Taki, Hirokazu Tsubone. J. Food Saf. Food Qual. 76(3):44132. 2025.

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・Evaluation of physiological functions and production performance in laying hens in three different housing systems. 

Gaku Shoji, Ryota Tochinai, Shin-ichi Sekizawa, Masayoshi Kuwahara*. Poultry Science. 104(8). 105256. 2025.

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・REPORT OF THE INTERNSHIP PROGRAM Embedding the 3Rs in Research Education and Practice from Classroom to Laboratory. Hsu JCN, Tochinai R. AAVS POST. the Asian Association of Veterinary Schools (AAVS). (2025) 5:18-19.

 

・REPORT OF THE INTERNSHIP PROGRAM Fostering Future Leaders in One Health: Short-Term Internship Program for University of the Philippines Students at the University of Tokyo.　

Tochinai R, Sekizawa S, Vitor RSJ, Flores M. AAVS POST. the Asian Association of Veterinary Schools (AAVS). (2025) 5:9-15.

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​・Loss of non-canonical translation initiation factors impairs perinatal cardiac function in mice. 

Takehiro Asai, Ryota Tochinai, Yoshiharu Tsuru, Marie Sekiguchi, Atsushi Minami, Wataru Fujii, Shigeru Kyuwa, Tetsuhiro Ogawa, and Shigeru Kakuta*. Exp Anim. 74(4):429-439. 2025.

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・Positive Behavioral, Morphophysiological, and Gene Expression Effects of the Administration of Virgin Coconut Oil in an Ischemic Stroke Surgical Rat Model. 

Vitor RSJ, Tochinai R, Sekizawa S, and Kuwahara M*. Int J Mol Sci. 26:6215. 2025.​

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・採卵鶏の生体機能に及ぼす誘導換羽の影響　~飼養形態の差異を含めた検討~.　

庄子 岳, 栃内 亮太, 関澤 信一, 桑原 正貴. 養鶏の友. 日本畜産振興会. 4:43-49. 2025.

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・Localized pulmonary vascular changes in a mouse model of subarachnoid hemorrhage

created by combining filament perforation and blood injection. 

Ryota Tochinai*, Takaya Suzuki, Kenji Tomita, Shin-ichi Sekizawa, Yoshinori Okada, Yasuyuki Taki, Masayoshi Kuwahara, Tatsushi Mutoh*. Microvasc Res. 160. 104810. 2025.

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・Nanoparticle O2 Carrier Composed of a Polymerized Stroma-Free Hemoglobin Core and Serum Albumin Shell as a Red Blood Cell Alternative in Hemorrhagic Shock Therapy. 

Kohyama, Natsumi; Takamine, Koki; Okamoto, Wataru; Yamada, Taiga; Yamaguchi, Masatoshi; Kohno, Mitsutomo; Tochinai, Ryota; Komatsu, Teruyuki*. ACS Appl Bio Mater. 8, 3, 2397–2407. 2025.

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・Protective role of protease-activated receptor-2 in anaphylaxis model mice. 

Maho Nakazawa, Ryota Tochinai, Wataru Fujii, Mao Komori, Tomohiro Yonezawa, Yasuyuki Momoi, Shingo Maeda*. PLOS ONE. 19(4): e0283915. 2024.

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・Diffusion MRI Fiber Tractography and Benzodiazepine SPECT Imaging for Assessing Neural Damage to the Language Centers in an Elderly Patient after Successful Reperfusion Therapy. 

Tatsushi Mutoh*, Yasuyuki Yoshida, Yasuko Tatewaki, Hongkun Chin, Ryota Tochinai, Junta Moroi, Tatsuya Ishikawa. Geriatrics. (2024) 9(2) 30.

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・Cardiopulmonary Events of the Elderly (≥75 Years) during Clazosentan Therapy after Subarachnoid Hemorrhage: A Retrospective Study from a Tertiary Stroke Center in Japan. 

Tatsushi Mutoh*, Hiroaki Aono, Wataru Seto, Takehiro Kimoto, Ryota Tochinai, Junta Moroi, Tatsuya Ishikawa. Medicina. 60(1):185. 2024.

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・Factors Influencing Discontinuation of Clazosentan Therapy in Elderly Patients with Aneurysmal Subarachnoid Hemorrhage: A Retrospective Study from a Japanese Single Center. 

Tatsushi Mutoh*, Hiroaki Aono, Wataru Seto, Takehiro Kimono, Ryota Tochinai, Junta Moroi, Tatsuya Ishikawa. Med Sci Monit. 30: e943303-1–e943303-7. 2024.

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・Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice.

Mutoh, Tatsushi*; Tochinai, Ryota; Aono, Hiroaki; Kuwahara, Masayoshi; Taki, Yasuyuki; Ishikawa, Tatsuya. Anim Models Exp Med. 7(1):77-81. 2024. 

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・Ivabradine ameliorates cardiomyopathy progression in a Duchenne muscular dystrophy model rat. 

Ryota Tochinai†*, Koichi Kimura†, Takeru Saika†, Wataru Fujii, Hiroyuki Morita, Koki Nakanishi, Yoshiharu Tsuru, Shin-ichi Sekizawa, Keitaro Yamanouchi, and Masayoshi Kuwahara. Exp Anim. 73(2). 145-153. 2024.

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・Anti-tumor and Cardiotoxic Effects of Microtubule Polymerization Inhibitors:The Mechanisms and Management Strategies. 

Tochinai R.*, Nagashima Y., Sekizawa S., Kuwahara M. J Appl Toxicol. 44. 96-106. 2024.　　

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・Report from AAVS Working Group - VETERINARY EDUCATION ENHANCEMENT (VEE) –. 

Nakagawa T, Tochinai R. AAVS POST. the Asian Association of Veterinary Schools (AAVS). (2023) 5:9-10.​

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・Polyoxazoline-Conjugated L-Asparaginase: An Antibody-Production-Free Therapeutic Agent for Acute Lymphoblastic Leukemia. 

Yamada, Taiga; Ishimaru, Marika; Shoji, Takuma; Tomiyasu, Hirotaka; Tochinai, Ryota; Taguchi, Kazuaki; Komatsu, Teruyuki*. ACS Appl Bio Mater. 6(12), 5789–5797, 2023.

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・Plasticity occurs in a specific phenotype of neurons in the nucleus tractus solitarius of dystrophin-gene mutated rats. 

Akitoshi Chikamoto, Ryota Tochinai, Shin-ichi Sekizawa*, Masayoshi Kuwahara. Eur. J. Neurosci. 1-16. 2023.​

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・Mastication stimuli regulate the heartbeat rate through rhythmic regulation by the hypothalamic-autonomic system; molecular and telemetric studies in weaning-stage rats. 

Seonmi Lee, Ryota Tochinai, Akihito Yasuoka*, Toshitada Nagai, Yoshikazu Saito, Masayoshi Kuwahara, Keiko Abe, and Tomiko Asakura. Front Neurosci. 2023; 17: 1260655.

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・Poly(2-ethyl-2-oxazoline)-Conjugated Hemoglobins as a Red Blood Cell Substitute. 

Wataru Okamoto, Yuuki Hiwatashi, Tatsuhiro Kobayashi, Yoshitsugu Morita, Hiroto Onozawa, Masayuki Iwazaki, Mitsutomo Kohno, Hirotaka Tomiyasu, Ryota Tochinai, Radostina Georgieva, Hans Bäumler, and Teruyuki Komatsu*. ACS Appl Bio Mater.​

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・Polyoxazoline-Conjugated Porcine Serum Albumin as an Artificial Plasma Expander for Dogs.

W. Okamoto, T. Usui, M. Hasegawa, T. Kobayashi, J. Fujisawa, K. Taguchi, K. Matsumoto, M. Kohno, M. Iwazaki, S. Shimano, I. Nagao, H. Toyoda, N. Matsumura, H. Tomiyasu, R. Tochinai, T. Komatsu*. Sci. Rep. 2023,13, 9512.

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・Co-administration of JQ1, a bromodomain-containing protein 4 inhibitor, enhances the antitumor effect of combretastatin A4, a microtubule inhibitor, while attenuating its cardiotoxicity. 

Orihara H., Ma M., Nagashima Y., Tochinai R.*, Sekizawa S., Kato D., Shinada M., Aoki S., Fujita N., Nakagawa T., Tsuru Y., Tatewaki Y., Mutoh T., Taki Y., Nishimura R., Kuwahara M. Biomed Pharmacother. 163, 114353. 2023.

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・Pretreatment of Tadalafil attenuates cardiotoxicity induced by combretastatin A4 disodium phosphate in rats. 

Nagashima Y., Tochinai R.*, Sekizawa S., Kato D., Nakagawa T., Tsuru Y., Tatewaki Y., Mutoh T., Taki Y., Kuwahara M. J Toxicol Pathol. 36, 151-158, 2023.

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・Preliminary study of heart rate variability in Criollo horses for the elucidation of their neurophysiological characteristics of autonomic nerve function. 

Seki N., Tochinai R.*, Sekizawa S., Márquez M.A., Fukuda K., Ohmura H., Kuwahara M. J Equine Sci. 34, 55-59. 2023.​

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・Favorable Effects of Virgin Coconut Oil on Neuronal Damage and Mortality after a Stroke Incidence in the Stroke-Prone Spontaneously Hypertensive Rat. 

Vitor R.J.S. II, Tochinai R., Sekizawa S., Kuwahara M*. Life, 12(11), 1857. 2022.

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・Direct Wiring of Liquid Metal on an Ultrasoft Substrate Using a Polyvinyl Alcohol Lift-off Method.

Koki Murakami, Ryota Tochinai, Daiki Tachibana, Yuji Isano, Ryosuke Matsuda, Fumika Nakamura, Yuta Kurotaki, Yutaka Isoda, Monami Yamane, Yuya Sugita, Junji Fukuda, Kazuhide Ueno, Norihisa Miki, Ohmi Fuchiwaki and Hiroki Ota*. ACS Appl Mater Interfaces. 14(5), 7241–7251. 2022.​

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・Loss of Group II Metabotropic Glutamate Receptor Signaling Exacerbates Hypertension in Spontaneously Hypertensive Rats. 

Hsu J.C., Sekizawa S*., Tochinai R., Kuwahara M*. Life. 11 (7), 720.​ 2021.

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・Chronic stimulation of group II metabotropic glutamate receptors in the medulla oblongata attenuates hypertension development in spontaneously hypertensive rats. 

Julia Chu-Ning Hsu, Shin-ichi Sekizawa*, Ryota Tochinai, Masayoshi Kuwahara*. PLOS ONE. 16(5). e0251495. 2021.

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・Autonomic nervous activity in rats can be evaluated by blood photoplethysmography-derived pulse rate variability analysis. 

Ryota TOCHINAI, Shin-ichi SEKIZAWA, Hidetoshi KOBAYASHI, Masayoshi KUWAHARA*. Translat Regulat Sci. 3(1):17 - 21. 2021.

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・Cardiac lesions in Duchenne muscular dystrophy model rats with out-of-frame Dmd gene mutation mediated by CRISPR/Cas9 system. 

Mao Miyamoto, Ryota Tochinai*, Shin-Ich Sekizawa, Takanori Shiga, Kazuyuki Uchida, Yoshiharu Tsuru, Masayoshi Kuwahara*. Journal of toxicologic pathology 33(4) 227 - 236. 2020.​

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・Effects of environmental enrichment on autonomic nervous activity in NSY mice. 

Kentaro Kaneko, Akitoshi Chikamoto, Julia Chu-Ning Hsu, Ryota Tochinai, Shin-Ichi Sekizawa, Machiko Yamamoto, Masayoshi Kuwahara*. Experimental animals 69(2) 161 - 167. 2019.

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・Early attenuation of autonomic nervous function in senescence accelerated mouse-prone 8

(SAMP8). 

Akitoshi Chikamoto, Shin-Ichi Sekizawa, Ryota Tochinai, Masayoshi Kuwahara*. Experimental animals 68(4) 511 - 517. 2019.

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・Histopathological and functional changes in a single-dose model of combretastatin A4 disodium phosphate-induced myocardial damage in rats. 

Ryota Tochinai*, Kayoko Komatsu, Junta Murakami, Yuriko Nagata, Minoru Ando, Chie Hata, Tomo Suzuki, Shoichi Kado, Toshihide Kobayashi, and Masayoshi Kuwahara. Journal of Toxicologic Pathology 31(4): 307–313. 2018.​​

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・Combretastatin A4 disodium phosphate-induced myocardial injury. 

Ryota Tochinai*, Yuriko Nagata, Minoru Ando, Chie Hata, Tomo Suzuki, Naoyuki Asakawa, Kazuhiko Yoshizawa, Kazumi Uchida, Shoichi Kado, Toshihide Kobayashi, Kimiyuki Kaneko, and Masayoshi Kuwahara. Journal of Toxicologic Pathology 29:163-171, 2016.

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・ラット腎臓の組織標本に発現した尿細管上皮細胞のアーティファクト軽減に関する固定液の検討.

永田 百合子*, 安藤 稔, 畑 千恵, 栃内 亮太, 角 将一. 実験病理組織技術研究会誌. (2015) 24:51-58.

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・Cardiotoxic changes of colchicine intoxication in rats: electrocardiographic, histopathological and blood chemical analysis.

Tochinai R*, Suzuki K, Nagata Y, Ando M, Hata C, Komatsu K, Suzuki T, Uchida K, Kado S, Kaneko K, Kuwahara M. J Toxicol Pathol. (2014) 27 (3-4):223-230.

 

​・Persistence and accumulation of micronucleated hepatocytes in liver of rats after repeated administration of diethylnitrosamine.

Narumi K*, Ashizawa K, Fujiishi Y, Tochinai R, Okada E, Tsuzuki Y, Tatemoto H, Hamada S, Kaneko K, Ohyama W. Mutat Res. (2013) 755(2):100-107.

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・Histopathological studies of microtubule disassembling agent-induced myocardial lesions in rats.　

Tochinai R*, Ando M, Suzuki T, Suzuki K, Nagata Y, Hata C, Uchida K, Kobayashi T, Kado S, Kaneko K. Exp Toxicol Pathol. (2013) 65(6):737-743.​